Table 1.
The influence of the force applied on an unconstrained dsDNA on hRad51 nucleoprotein filament polymerization
| Force during incubation (pN) | Percentage of hRad51 filament (%) | Initial polymerization velocity (nm s−1) |
|---|---|---|
| 0.5 ± 0.1 | 65 ± 5 | 2.1 ± 0.1 (69 ± 1 monomers min−1) |
| 2.8 ± 0.2 | 74 ± 5 | 5.0 ± 0.1 (520 ± 10 monomers min−1) |
| 3.0 ± 0.3 | 87 ± 5 | 6.2 ± 0.1 (640 ± 10 monomers min−1) |
| 4.0 ± 0.4 | 82 ± 5 | 6.3 ± 0.1 (660 ± 10 monomers min−1) |
| 6.0 ± 0.6 | 78 ± 5 | 7.8 ± 0.2 (840 ± 20 monomers min−1) |
| 7.0 ± 0.7 | 79 ± 5 | 14.5 ± 0.4 (1550 ± 40 monomers min−1) |
We measured the initial polymerization velocity and the final filament length for each force, and calculated the relative extension (percentage of coverage by active filament) as described in the Results section.