Table 4.
The percentage of ssDNA length covered by hRad51 and the initial velocity of polymerization under different experimental conditions
| Buffer type | [hRad51] varies Force is fixed (6pN) | [hRad51] is fixed = 3 µM Forces varies | |||
|---|---|---|---|---|---|
| 200 nM | 3 µM | 2.0 ± 0.2 pN | 4.0 ± 0.4 pN | 6.0 ± 0.6 pN | |
| ATP, A buffer (2 mM Mg2+) | 13 ± 5% | 24 ± 5% | – | – | – |
| 7.4 ± 0.2 nm s−1 | 44.2 ± 0.2 nm s−1 | ||||
| 700±20 monomers min−1 | 4210 ± 20 monomers min−1 | ||||
| ATP, B buffer (5 mM Ca2+) | 36 ± 5% | 88 ± 5% | 24 ± 5% | 67 ± 5% | 88 ± 5% |
| 12.8 ± 0.2 nm s−1 | 80 ± 10 nm s−1 | 13 ± 1 nm s−1 | 80 ± 10 nm s−1 | 80 ± 10 nm s−1 | |
| 1220 ± 20 monomers min−1 | 8000 ± 1000 monomers min−1 | 1100 ± 90 monomers min−1 | 7400 ± 900 monomers min−1 | 8000 ± 1000 monomers min−1 | |
The error bars are given by the fit for each individual experiment. Each case of the table contains three lines: Line 1: percent coverage; Line 2: polymerization rate in nm/s; Line 3: Polymerization rate in monomers per min.