Table 6.

Summary of the data on hRad51 nucleoprotein filament assembly/disassembly and mechanical properties

	hRad51
	ssDNA	DsDNA
Persistence length with ATP (WLC)	360 ± 30 nm	390 ± 150 nm
Persistence length with ATPγS (WLC)	670 ± 30 nm	270 ± 80 nm
Average percent coverage in the presence of ATP	88 ± 2% (3 µM hRad51, 6 pN)	82 ± 2% (200 nM hRad51, 6 pN)
Average percent coverage in the presence of AMP-PNP	72 ± 5% (3 µM hRad51, 6 pN)	45 ± 16% (200 nM hRad51, 6 pN)
Average percent coverage in the presence of ATPγS	72 ± 3% (3 µM hRad51, 6 pN)	35 ± 4% (200 nM hRad51, 6 pN)
Initial polymerization velocity in the presence of ATP	80 ± 10 nm s−1	7.8 ± 0.2 nm s−1
(nm s−1 and monomers min−1)	8000 ± 1000 monomers min−1 (3 µM hRad51, 6 pN)	840 ± 20 monomers min−1 (200 nM hRad51, 6pN)
Depolymerization velocity in the presence of ATP	450 monomers min−1 (6 pN)	3 monomers min−1 (6 pN)
Cooperativity during polymerization, with ATP (number of monomers involved in a nucleus)	–	5.5 ± 1.5