Table 4.

Flow cytometry analysis of two MCF7 and MDA-MB-468 tumor cell lines and normal breast epithelial MCF10-2A cells. Irrelevant antibody anti-SP2 was used as negative control, while an α-tubulin monoclonal antibody was used as positive control for intracellular staining

Cell line/scFv antibody	Surface staining of alive cells			Intracellular staining
MCF7	% pos	MFI*	iMFI**	% pos	MFI	iMFI
α-tubulin	n.t.	n.t.	n.t.	88.04	684.49	60262.5
anti-SP2	2.73	24.45	66.7	0.36	4221.17	1519.6
MIX7	2.84	29.19	82.9	73.03	603.58	44079.4
MIX17	2.68	22.76	59.9	84.2	733.63	61771.6
MIX39	3.98	22.9	91.1	13.89	504.85	7012.4
MDA-MB-468	% pos	MFI	iMFI	% pos	MFI	iMFI
α-tubulin	n.t.	n.t.	n.t.	85.22	259.18	22087.3
anti-SP2	1.9	51.17	97.2	0.98	579.13	567.5
MIX7	3.93	40.4	158.8	37.14	214.39	7962.4
MIX17	5.38	33.04	177.8	70.54	260.36	18365.8
MIX39	3.33	30.04	100.0	2.51	237.64	596.5
MCF10-2A	% pos	MFI	iMFI	% pos	MFI	iMFI
α-tubulin	n.t.	n.t.	n.t.	42.05	169.64	7133.4
anti-SP2	0.44	95.68	42.1	0.44	1515.5	666.8
MIX7	0.94	208.51	196.0	0.29	962.79	279.2
MIX17	0.81	11.84	9.6	1.06	824.83	874.3
MIX39	0.93	175.42	163.1	6.49	156.57	1016.1

* mean fluorescence intensity

** integrated MFI (% pos × MFI)

n.t.- not tested