Table 7.
Amplification of transcripts by classical PCR | |
Primers | Sequence |
ExosqF | 5' TGC AGG CGA TGC GAG TGC 3' |
ExosqR | 5' GCT ACA ACA GCG GTT AGG 3' |
GalaxinF | 5' ATT TCG TTT GCA ACA GCC 3' |
GalaxinR | 5' CTT CCT CTG CAG CAC TGG 3' |
ChPrF | 5' AAT TCT GAG ACC GGT GAC C 3' |
ChPrR | 5' TCC AAG ACC GTG TTG AGC 3' |
RpCAtrF | 5' TAC AAA GAT CCA ATC CAG C 3' |
RpCAtrR | 5' ACG AGG ACG ACA CCT GG 3' |
RpCAbrF | 5' TAC AAG GAT GCC ATT AGC 3' |
RpCAbrR | 5' AGA GCA GCA GAC CTT ACG 3' |
IntraGlobF | 5' GGA AAG GAC GTC GAC AGC 3' |
IntraGlobR | 5' TGC TGC TTG GTT AGT CCC 3' |
MVPF | 5' GAG AAC AGA ATG ACA TGG 3' |
MVPR | 5' TTT CAC CTG CAT CTC GGG 3' |
ccoxIF | 5' ACA GGT TTA GTA GCC ACT 3' |
ccoxIR | 5' GTG TTG ATA TAG GAC TGG 3' |
Amplification of transcripts by quantitative PCR | |
Primers* | Sequence |
RpCAbrFq | 5' TGG TTT CAC CCC GTC GAA 3' |
RpCAbrRq | 5' GGT CTG GTC TTT TCT CGC CAT A 3' |
RpCAtrFq | 5' GCC AGG TGT CGT CCT CGT T 3' |
RpCAtrRq | 5' TCA CAA ATG TCC AGT GCC AGT T 3' |
16SFq | 5' CGT AAG ACT ATA GCT GGT TTT CCA AA 3' |
16SRq | 5' TTA TCA AAG ATT TTT TCT TGG TTC ACT AAT 3' |
MVPFq | 5' GAT TGA GAC AAC CAA GTT CAG GAA 3' |
MVPRq | 5' CTG GCG ATT GCC TGA ATT G 3' |
CathepFq | 5' TAC ATG GCC CGT AAT AAG GAC A 3' |
CathepRq | 5' GCT GGC TTG TGA TGC AAC AC 3' |
ccoxIFq | 5' CTA ATG GGA GGC TTC GGA AAC 3' |
ccoxIRq | 5' AGG TGC CCC TAG CAT TAA AGG 3' |
ATPF1Fq | 5' TGC AGG ACA TCA TTG CCA TC 3' |
ATPF1Rq | 5' TGT CCT CCT GGG ACA ACT CG 3' |
ChPrFq | 5' GTC GTC GGA ATG GCG AGT TA 3' |
ChPrRq | 5' AGC GTT GCT GGC TGT TTT G 3' |
MHFq | 5' AGA GGC ACA CCA ACA ACC GT 3' |
MHRq | 5' CCC GAT TGG TTC ATC ACA GC 3' |
TCRFq | 5' AAT CCG ACG TGG CGA TCA T 3' |
TCRFq | 5' GGT CAT TGT TGT TGC CTG GG 3' |
TRbwC27Fq | 5' CGA CGG TGG TAC CCC GTA TA 3' |
TRbwC27Rq | 5' CCG CAA CCT TTG AAC CTC AG 3' |
*Primers designed by Primer Express software (ABI PRISM™)