Fig. 5.

RTL treatment ongoing axonal injury in the lumbar spinal cord of EAE mice. Left panel: Immunoblotting result showing that RTL401 treatment reversed the development of axonal injury as indicated by abnormal expression of non-phosphorylated neurofilaments in lumbar spinal cords from mice with EAE. EAE mice were euthanized by perfusion with PBS at disease onset (day 11, disease score 1.5), peak (between day 15 and day 20, when disease scores reached 4.5), or at the termination of the experiment (day 60 of EAE, after RTL401 or vehicle treatment). The lysates of whole lumbar spinal cords from each group (4 PBS-perfused mice from a group of 8) were pooled and the amount of NPNFL was detected after immunoblotting. Each column represents pooled samples from 4 mice from a single group. Right panel: Immunoblotting results showed the amount of NPNFL in the lumbar spinal cord samples from three randomly chosen mice (3 out of the 4 samples in the pool) from naïve, vehicle-treated (day 60 of EAE) or RTL401-treated (day 60 of EAE) groups. Each band represents a lumbar spinal cord sample from an individual mouse. The experiment was repeated two times.