Table 6.
ABI 3100 Genetic Analyzer Electrophoresis Conditions for the Common Platform Using the POP6 Polymer and a 36 cm Array and Data Collection Software v2.0
| Parameter | Data collection |
|---|---|
| Shorter [5–70 (CGG)n] repeats | |
| Running temperature | 60°C |
| Cap fill volume | NA |
| Current tolerance | NA |
| Run current | NA |
| Voltage tolerance | NA |
| Pre-run voltage | 15 kV |
| Pre-run time | 180 seconds |
| Injection voltage | 10 kV |
| Injection time | 1 second |
| Run voltage | 15 kV |
| Number of steps | 10 nK |
| Voltage step interval | 60 seconds |
| Data delay time | 300 seconds |
| Run time | 3000 seconds |
| Longer [71–230 (CGG)n] repeats | |
| Running temperature | 60°C |
| Cap fill volume | NA |
| Current tolerance | NA |
| Run current | NA |
| Voltage tolerance | NA |
| Pre-run voltage | 15 kV |
| Pre-run time | 180 seconds |
| Injection voltage | 15 kV |
| Injection time | 22 seconds |
| Run voltage | 15 kV |
| Number of steps | 10 nK |
| Voltage step interval | 60 seconds |
| Data delay time | 300 seconds |
| Run time | 5600 seconds |