Table 3.
Specimen Failure Rates
Sample source | Extraction method | T4 failure |
---|---|---|
CSF | MagNAPure Compact | 5* of 169 (<3%) |
CSF | BioRobot M48 | 0 of 36 |
Serum | MagNAPure Compact | 0 of 18 |
Stool | Manual QIAGEN | 3† of 67 (4.4%) |
Repeated successful extraction and testing of one specimen revealed the initial T4 failure was due to extraction failure.
Nucleic acids were diluted 1:10 and retested; T4 bacteriophage result was positive, demonstrating that failure was due to the presence of PCR inhibitors.