Table 2.

Sequences of PCR Primers and Hybridization Probes

Oligo		Sequence	Positions (nucleotides)	GenBank accession no.
KIT primers	1F	5′-CACAGAGACTTGGCAGCCAG-3′	7097-7116	L04143
	1B	5′-CAGGATTTACATTATGAAAGTCACGG-3′	7309-7284
	2F	5′-CAGCCAGAAATATCCTCCTTACT-3′	7110-7132
	2B	5′-TTGCAGGACTGTCAAGCAGAG-3′	7247-7227
KIT probes	Sensor	5′-AGCCAGAGTCATCAAGAATGATTCTA-3′(Fl)*	7168-7193
	Anchor	(LC)5′-ATGTGGTTAAAGGAAACGTGAGTACCCA(p)-3′†	7197-7214
	PNA	5′-GCCAGAGACATCAAGAATG-3′	7169-7187
JAK2 primers	1F	5′-GGCAGAGAGAATTTTCTGAAC-3′	54,915-935	AL161450
	1B	5′-CCTAGCTGTGATCCTGAAACTGA-3′	55,164-142
	2F	5′-AGCAGCAAGTATGATGAGC-3′	55,000-018
	2B	5′-AGCTGTGATCCTGAAACTGAATTTTCT-3′	55,161-135
JAK2 probes	Sensor	5′-GTCTCCACAGAAACATACTCCATAA-3′(Fl)*	55,072-048
	Anchor	(LC)5′-TAAAACCAAATGCTTGTGAGAAAGCTTGCT-3′(p)†	55,045-016
	LNA	5′-CCACAGACACATACTCCAp-3′	55,068-062

^*The sensor probes were 3′-fluorescein-labeled (Fl).

^†The anchor probes were 5′-terminal labeled with LightCycler Red 640 (LC) and 3′-terminal blocked with a phosphate group (p).