Table 1.
Primers and thermal profiles for real-time RT-PCR.
| Gene | GenBank Accession No. | Primer | Product size (bp) | Annealing temp./time (°C/s) | Extension temp./time (°C/s) |
| β-actin | NM_007393 | 5'-tgttaccaactgggacgaca-3' 5'-ggggtgttgaaggtctcaaa-3' |
165 | 55/7 | 72/20 |
| Bmp4 | NM_007554 | 5'-tgatacctgagaccgggaag-3' 5'-agccggtaaagatccctcat-3' |
190 | 55/8 | 72/20 |
| Msx1 | NM_010835 | 5'-agctctgctgccctatacca-3' 5'-cttggcctctgcatccttag-3' |
205 | 57/8 | 72/20 |
| Pax3 | NM_008781 | 5'-ctgcactcaagggactcctc-3' 5'-gtgaaggcgagacgaaaaag-3' |
169 | 57/7 | 72/20 |