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. 2007 Oct 29;8:90. doi: 10.1186/1471-2202-8-90

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Copyright © 2007 Pellissier et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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12 μm sagittal brain sections of a two-month-old rat were processed for immunohistochemistry. The sections were incubated with anti-Necl-3 antibody, which was revealed with a secondary antibody coupled to Alexa 594. Nuclei were visualized with DAPI. Panel A (average projection of 64 z-stacks of 0.12 micron each) shows part of the third ventricle (asterisk) bordered by the ependymal cell layer. Panel B (average projection of 57 z-stacks of 0.12 micron each) shows ependymal cells of the choroid plexus in the third ventricle.