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The Fc-Necl-3 fusion protein (N3) or IgGs (IgG) were immobilized on protein A beads. These were used for pull-downs in lysates of S2 cells transfected either with a control vector (mock) or with Necl-2-GFP. After the pull-downs, the proteins retained on the beads were subjected to SDS-PAGE (12%), followed by western blotting with an anti GFP mAb. 15% of the input lysate material was loaded on the same gel (input).
