Table 1.
Biochemical parameters of vertebrate PGI proteins.
| Biochemical characters1 | No. of hydrophilic charged residues | |||||
| No. of amino acids | Hydro-phobicity (GRAVY) | Isoelectric point (pI) | Positively charged (Arg+Lys) | Negatively charged (Asp+Glu) | Difference2 | |
| Teleost fish PGI-1 | ||||||
| Fugu-1 | 552 | -0.261 | 6.33 | 58 | 64 | -6 |
| Mullet-1 | 553 | -0.282 | 6.30 | 57 | 63 | -6 |
| Smelt-1 | 553 | -0.284 | 6.21 | 54 | 62 | -8 |
| Zebrafish-1 | 553 | -0.265 | 6.45 | 53 | 58 | -5 |
| Eel-1 | 553 | -0.359 | 6.36 | 57 | 63 | -6 |
| Arowana-1 | 553 | -0.277 | 6.22 | 56 | 64 | -8 |
| Teleost fish PGI-2 | ||||||
| Fugu-2 | 553 | -0.294 | 6.96 | 59 | 61 | -2 |
| Mullet-2 | 553 | -0.265 | 7.85 | 61 | 60 | 1 |
| Smelt-2 | 552 | -0.337 | 7.36 | 64 | 64 | 0 |
| Zebrafish-2 | 553 | -0.304 | 6.82 | 59 | 61 | -2 |
| Eel-2 | 553 | -0.285 | 6.75 | 58 | 61 | -3 |
| Arowana-2 | 553 | -0.280 | 7.07 | 63 | 64 | -1 |
| Non-teleost fish PGI | ||||||
| Sturgeon | 555 | -0.356 | 6.82 | 59 | 61 | -2 |
| Gar | 555 | -0.308 | 6.83 | 59 | 61 | -2 |
| Amia | 555 | -0.355 | 6.62 | 60 | 63 | -3 |
| Bichir | 556 | -0.278 | 6.84 | 58 | 60 | -2 |
| Tetrapod PGI | ||||||
| Toad | 553 | -0.226 | 7.68 | 59 | 58 | 1 |
| Snake | 553 | -0.237 | 8.72 | 62 | 57 | 5 |
| Chicken | 553 | -0.255 | 8.34 | 64 | 61 | 3 |
| Mouse | 558 | -0.294 | 7.75 | 61 | 60 | 1 |
| Pig | 558 | -0.340 | 7.79 | 62 | 61 | 1 |
| Rat | 558 | -0.285 | 7.38 | 61 | 61 | 0 |
| Hamster | 558 | -0.322 | 7.08 | 59 | 60 | -1 |
| Rabbit | 558 | -0.292 | 7.11 | 58 | 59 | -1 |
| Human | 558 | -0.344 | 8.42 | 62 | 59 | 3 |
| Jawless fish PGI | ||||||
| Hagfish | 554 | -0.238 | 7.82 | 57 | 56 | 1 |
1The predicted overall hydrophobicity (GRAVY; grand average of hydropathicity) and pI values of the PGI proteins were estimated based on the amino acid sequences translated from the cDNA sequences of Pgi genes using the ProtParam tool [49]
2Differences in number of positively and negatively charged residues in each PGI protein