Fig. 3.

Phylogenetic network showing relationships between var gene DBLα sequence tags transcribed by P. falciparum isolates, generated using Neighbour-Net [35]. Sequences transcribed by isolates from African children with cerebral malaria (CM, red), hyperparasitaemia (HYP, blue), and uncomplicated malaria (U, green) are compared to a selection of var genes from the laboratory clone 3D7. For the 3D7 genes, the gene name is preceded by A, B, C, BA or BC to indicate the group to which the gene belongs. The sequences fall into two major clades, with the DBLα0-like sequences to the left of the dotted line and the DBLα1-like sequences to the right. For the predominant gene from nine of the Malian isolates, the var gene upstream region was determined by PCR and is indicated as a boxed letter.