Fig. 8.

Growth and development phenotypes on bacterial lawns. (A) Plaque edge phenotype of wild-type and gα4⁻ cells with chimeric Gα subunit vectors on bacterial lawns. Wild-type cells without Gα subunit (none) expression vector or wild-type and gα4⁻ cells with the Gα2/4 subunit (Gα2/4) or Gα5/4 subunit (Gα5/4) expression vector were placed on a bacterial lawns and allowed to form plaques. Plaque edges were photographed after 4 days using the same magnification (20X) on a dissecting microscope. (B) Wild-type cells with the Gα5/4 subunit expression vector developing within a plaque on a bacterial lawn. Developing aggregates were photographed using a dissecting microscope (40X). Arrows indicate mature fruiting body formation.