Figure 4.

Effect of VEGF and SU5416 on endothelial cell growth. Ovine pulmonary artery endothelial cells (PAECs) from normal lambs and lambs with persistent pulmonary hypertension of the newborn (PPHN) were grown in 5% fetal bovine serum under 3% oxygen conditions with and without VEGF (50 ng/ml) and SU5416 (10 μM). (A) VEGF treatment stimulated growth of PAECs in both normal and PPHN cells (solid bars, control; open bars, VEGF). (B) VEGF receptor blockade with SU5416 decreased growth in both normal and PPHN PAECs (solid bars, control; open bars, SU5416). Error bars represent SD from mean.