TABLE 1.
SUMMARY OF THE PROPORTION OF CELLS EGFP-POSITIVE IN AIRWAY AND ALVEOLAR TISSUES AFTER INTRATRACHEAL ADMINISTRATION OF LENTIVIRAL SUPERNATANT
EGFP Analysis of Airway Cells
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EGFP Analysis of All Alveolar Cells
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Animal | Vector | Cells Counted* | Number of GFP+CC10+ | Cells Counted† | Number of GFP+proSPC+ | % GFP+ Cells |
IT1-8 | CC10 | 422 | 10 | 2.4 | ||
IT4-11 | CC10 | 496 | 18 | 3.6 | ||
IT2-2 | SPC | 638 | 16 | 2.5 | ||
IT2-5 | SPC | 966 | 22 | 2.3 | ||
IT3-2 | SPC | 3,914 | 178 | 4.6 |
Definition of abbreviations: CC10, Clara cell 10-kD protein; EGFP, enhanced green fluorescent protein; GFP, green fluorescent protein; SPC, surfactant protein C.
Exclusively airway cells were counted for this analysis.
All cells within in the alveolar region were counted for this analysis, and thus includes AECI, AECII, as well as vascular and hematopoietic cells.