TABLE 1.
DYNAMIC SURFACE TENSION LOWERING OF DEPN-8 + 1.5% MIXED BOVINE SURFACTANT PROTEIN-B/C AND CALF LUNG SURFACTANT EXTRACT WITH AND WITHOUT INHIBITORS
| Surface Tension (mN/m) at Minimum Bubble Radius at Time (min)
|
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|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Surfactant Mixture | 0.25 | 0.5 | 1 | 2 | 5 | 10 | 15 | 20 | ||||||||
| 0.5 mg/ml surfactant | ||||||||||||||||
| DEPN-8 + 1.5% SP-B/C | 13 ± 0 | 9 ± 1 | 6 ± 1 | 4 ± 1 | 1 ± 1 | < 1 | — | — | ||||||||
| + 0.1 U PLA2 | 16 ± 2 | 9 ± 1 | 6 ± 1 | 4 ± 1 | 1 ± 1 | < 1 | — | — | ||||||||
| + 15% (by wt) LPC | 35 ± 1 | 34 ± 1 | 31 ± 1 | 29 ± 1 | 27 ± 1 | 25 ± 1 | 22 ± 0 | 20 ± 0 | ||||||||
| + 3.0 mg/ml BSA | 48 ± 1 | 46 ± 1 | 40 ± 2 | 28 ± 1 | 22 ± 1 | 18 ± 1 | 11 ± 1 | 6 ± 1 | ||||||||
| + 1 mM peroxynitrite | 48 ± 1 | 45 ± 1 | 40 ± 1 | 32 ± 1 | 24 ± 1 | 20 ± 1 | 19 ± 1 | 18 ± 1 | ||||||||
| CLSE | 18 ± 0 | 15 ± 1 | 11 ± 2 | 9 ± 2 | 7 ± 2 | < 1 | — | — | ||||||||
| + 0.1 U PLA2 | 26 ± 2 | 24 ± 1 | 23 ± 1 | 23 ± 1 | 21 ± 1 | 20 ± 1 | 19 ± 0 | 19 ± 1 | ||||||||
| + 15% LPC | 35 ± 0 | 35 ± 0 | 31 ± 0 | 29 ± 0 | 27 ± 0 | 26 ± 0 | 25 ± 0 | 24 ± 0 | ||||||||
| + 3.0 mg/ml BSA | 46 ± 1 | 43 ± 1 | 36 ± 1 | 29 ± 2 | 22 ± 2 | 18 ± 1 | 12 ± 1 | 7 ± 1 | ||||||||
| + 1 mM peroxynitrite | 49 ± 1 | 44 ± 2 | 39 ± 1 | 33 ± 1 | 25 ± 1 | 22 ± 1 | 21 ± 1 | 20 ± 1 | ||||||||
| 2.5 mg/ml surfactant | ||||||||||||||||
| DEPN-8 + 1.5% SP-B/C | 12 ± 0 | 8 ± 1 | 6 ± 1 | 2 ± 0 | < 1 | — | ||||||||||
| + 0.1 U PLA2 | 15 ± 3 | 8 ± 1 | 5 ± 1 | 3 ± 0 | < 1 | — | ||||||||||
| + 15% LPC | 33 ± 2 | 30 ± 1 | 28 ± 1 | 20 ± 1 | 11 ± 1 | 4 ± 0 | < 1 | |||||||||
| + 3.0 mg/ml BSA | 21 ± 0 | 17 ± 1 | 13 ± 0 | 8 ± 0 | 4 ± 0 | < 1 | — | |||||||||
| + 1 mM peroxynitrite | 18 ± 1 | 15 ± 1 | 11 ± 1 | 7 ± 1 | 2 ± 1 | < 1 | — | |||||||||
| CLSE | 5 ± 2 | < 1 | — | — | — | — | — | — | ||||||||
| + 0.1 U PLA2 | 24 ± 2 | 23 ± 1 | 20 ± 1 | 19 ± 2 | 15 ± 1 | 14 ± 1 | 14 ± 1 | 14 ± 0 | ||||||||
| + 15% LPC | 34 ± 1 | 32 ± 1 | 29 ± 1 | 26 ± 1 | 19 ± 1 | 12 ± 1 | 5 ± 0 | <1 | ||||||||
| + 3.0 mg/ml BSA | 21 ± 1 | 17 ± 1 | 14 ± 0 | 10 ± 0 | 3 ± 0 | < 1 | — | — | ||||||||
| + 1 mM peroxynitrite | 22 ± 0 | 20 ± 1 | 18 ± 1 | 12 ± 2 | 5 ± 1 | < 1 | — | — | ||||||||
Definition of abbreviations: BSA, bovine serum albumin; CLSE, calf lung surfactant extract; LPC, C18:1 lysophosphatidylcholine; PLA2, phospholipase A2; SP, surfactant protein.
Data are mean ± SEM for n = 4–5. Surface tension at minimum radius (minimum surface tension) is shown as a function of time of pulsation on a bubble surfactometer (37°C, 20 cycles/min, 50% area compression, 0.5 or 2.5 mg phosphonolipid or phospholipid/ml). Mixed surfactant proteins SP-B/C were column purified from CLSE (see Materials and Methods).