Table 2.
locus | forward primera | reverse primer | annealing T (°C) |
---|---|---|---|
HvA10 | CACGACGTTGTAAAACGACGAAAAGGTCAAAGGTCACAACGAC | TTGCCCTGGATGCCATACAG | 50 |
HvH07 | CACGACGTTGTAAAACGACCTCGTAAGAAATGGAAACTG | GATGTTGAAACCCGAAGC | 55 |
Hm8 | CACGACGTTGTAAAACGACTGGATGATAATGCGTCGTCAGTC | TCTTTGGTTCACAGGGCTTAGTTG | 55 |
Hm10 | CACGACGTTGTAAAACGACCTCTCCACAGCCTTATGGTGTTG | AGGATGAGGTGAAGCGGGGATG | 55 |
Hm12 | CACGACGTTGTAAAACGACGCACCAAGCCAGAATAAGGTTAG | TCTCCACTTTCACTCCCGCTC | 50 |
Hv351 | CACGACGTTGTAAAACGACCGCAATCTGCCTGAAAAAGTAAAC | CGAATGTGTTGGATGCCACTAAC | 50 |
HvT379 | CACGACGTTGTAAAACGACATTGGGAACAAGAACAAGCC | CAGAAGGTGGAGAAGTGGTTG | 50 |
Hm1 | CACGACGTTGTAAAACGACTCAGGCGACATCCTCTTCATCG | ATGGCTACCACTGCGTTGTTG | 50 |
Hm2 | CACGACGTTGTAAAACGACAGGAACTCATCATTTATCTCCAGCC | ACGGAAACCACGGTCTCCAAGAG | 50 |
HvD7 | CACGACGTTGTAAAACGACTCTACTTCCAGAACTCGTG | GCAGCCTTTACGGAGCGG | 50 |
The first 19 nt of each forward primer corresponds to a fluorescently labelled M13 primer used in the reaction.