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. 2007 Apr 10;274(1617):1481–1487. doi: 10.1098/rspb.2007.0248

Table 2.

Microsatellite loci, primers and annealing temperatures.

locus forward primera reverse primer annealing T (°C)
HvA10 CACGACGTTGTAAAACGACGAAAAGGTCAAAGGTCACAACGAC TTGCCCTGGATGCCATACAG 50
HvH07 CACGACGTTGTAAAACGACCTCGTAAGAAATGGAAACTG GATGTTGAAACCCGAAGC 55
Hm8 CACGACGTTGTAAAACGACTGGATGATAATGCGTCGTCAGTC TCTTTGGTTCACAGGGCTTAGTTG 55
Hm10 CACGACGTTGTAAAACGACCTCTCCACAGCCTTATGGTGTTG AGGATGAGGTGAAGCGGGGATG 55
Hm12 CACGACGTTGTAAAACGACGCACCAAGCCAGAATAAGGTTAG TCTCCACTTTCACTCCCGCTC 50
Hv351 CACGACGTTGTAAAACGACCGCAATCTGCCTGAAAAAGTAAAC CGAATGTGTTGGATGCCACTAAC 50
HvT379 CACGACGTTGTAAAACGACATTGGGAACAAGAACAAGCC CAGAAGGTGGAGAAGTGGTTG 50
Hm1 CACGACGTTGTAAAACGACTCAGGCGACATCCTCTTCATCG ATGGCTACCACTGCGTTGTTG 50
Hm2 CACGACGTTGTAAAACGACAGGAACTCATCATTTATCTCCAGCC ACGGAAACCACGGTCTCCAAGAG 50
HvD7 CACGACGTTGTAAAACGACTCTACTTCCAGAACTCGTG GCAGCCTTTACGGAGCGG 50
a

The first 19 nt of each forward primer corresponds to a fluorescently labelled M13 primer used in the reaction.