Figure 9. Mutations immediately upstream from the GREcore –327b1 site leading to a complete GRE site resulted in full responsiveness of AE2 alternate promoter to either UDCA or dexamethasone alone.
Luciferase activities of PLC/PRF/5 cells transiently transfected with construct I-b1 or I-b1GRETat — the latter having the complete sequence of the GRE site in the rat Tat promoter, as depicted in Figure 5 — and treated for 24 hours with UDCA and/or dexamethasone. Values of luciferase activity (normalized with Renilla values) are given as fold activity relative to the activity in cells transfected with construct I-b1 in the absence of UDCA and DEX (indicated by a vertical arrow). Data are mean ± SD; n = 9 each.