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. 2008 Feb 1;118(2):515–525. doi: 10.1172/JCI33304

Figure 4. SMCs populating the DA of Myocd conditional mutant mice exhibit markedly diminished expression of SMC contractile proteins.

Figure 4

(A and B) H&E-stained section of the DA of MyocdF/F control mouse (A) and MyocdF/F/Wnt1-Cre+ mutant mouse (B). (C, E, and G) Immunohistochemical analyses of the DA of E16.5 MyocdF/F control mouse demonstrating robust expression of SMA (green staining) (C), SM-MyHC (red staining) (E), and SM22α (green staining) (G). (D, F, and H) By contrast, expression of SMA (D), SM-MyHC (F), and SM22α (H) is markedly diminished in the DA of E16.5 MyocdF/F/Wnt1-Cre+ mutant mice. (I and J) X-gal–stained section of P2 MyocdF/F/Wnt1-Cre+/R26R+ triple mutant mice revealed that essentially all SMCs populating the DA express β-galactosidase (blue stain), confirming that these cells are of neural crest origin. Original magnification, ×100 (A–I); ×200 (J).