Table 1.
Primers and PCR conditions. (The new primers designed for this study include the indication of the position of their 3′ end within the dog complete mitochondrial sequence (Kim et al. 1998). Ta, annealing temperature; Cb, cytochrome b; D, partial D-loop (and flanking tRNA sequences); F, forward; R, reverse.)
| primer name | sequence (5′–3′) | Ta | reference |
|---|---|---|---|
| Cb-M1-F | CTC ACA TGG AAT CTA ACC ATG AC | 56 | Kurose et al. (2000) |
| Cb-L14474-F | CTT TAT CTG CCT ATT CCT ACA CG | 56 | this study |
| Cb-H14549-R | CTA TGA ATG CAG TTG CTA TGA C | 55 | this study |
| Cb-Mustela07-F | TTC ATC ATT TCA GCA CTA GCA GCA GTC | 55 | Fleming & Cook (2002) |
| Cb-Mustela06-R | GTG GAA TGG GAT TTT GTC AGA GTC GGA | 55 | Fleming & Cook (2002) |
| Cb-L14897-F | GCC CTA TTC CTT ATT CTA ACA C | 55 | this study |
| Cb-H14939-R | GGC TGG GAT ATA GTT GTC TGG | 56 | this study |
| D-Cbz-F | ATG AAT TGG AGG ACA ACC AGT | 55 | Kurose et al. (1999) |
| Cb-MR1-R | TCT TCC TTG AGT CTT AGG GAG | 56 | Kurose et al. (2000) |
| D-L15454-F | CTG ACA TTC TAA CTA AAC TAT TCC | 55 | this study |
| D-H15777-R | TGA AGT AAG AAC CAG ATG CCA G | 55 | this study |
| D-MER-R | CGC GGG TGG TGT ATA AAT AT | 55 | Kurose et al. (1999) |