Figure 1.

GSH content of the different yeast strains. The parental yeast strain W303a as well as W303a in which the γECS gene had been disrupted with LEU2 and transformed with the plasmids (clones 1 and 4) were grown in liquid minimal medium for 36 h at 30°C. Cells were sedimented by centrifugation and GSH was extracted and measured as described in Materials and Methods. GSH content of the disruption mutant YΔγECS was undetectable by HPLC analysis. GSH content in the extracts is expressed as nmol/10⁹ cells. The supplements (100 μM) to the medium are: C, Cys; M, Met; O, O-acetyl Ser; T, thiosulfate. Bars represent means ± SE (n = 3).