Table 1.

Glutathione biosynthetic enzyme activity in yeast strains

Strain*	γECS activity†	GSHS activity†
W303a	11.48  ±  1.85	50.32  ±  2.39
Clone 1	1.86  ±  0.02	19.47  ±  1.88
Clone 4	2.72  ±  0.51	23.46  ±  1.18
Mutant	0.59  ±  0.00	22.05  ±  0.16

^*W303a, parental strain; clone 1 and clone 4, mutant strains transformed with the plasmids harboring the corresponding cDNAs; mutant, W303a in which the γECS gene has been disrupted with the LEU2 gene. 

^†γECS and GSHS activity in extracts of the respective yeast strains expressed as nmol product/min per mg protein. Values are the means of three experiments ± SE.