Figure 3.

Delayed-phase PGD₂ generation. Delayed-phase PGD₂ generation (A) was determined in BMMC derived from BALB/c, cPLA₂+/+, and cPLA₂−/− mice. BMMC were stimulated with SCF + IL-10 + IL-1β for 1 h, washed, and incubated for 7 h more with the same cytokines. PGD₂ released into the supernatant was measured by using RIA. PGHS-2 induction (B) was assessed by SDS/PAGE immunoblotting 8 h after the initial activation with SCF + IL-10 + IL-1β (+) or after continued culture in WEHI-3 cell-conditioned medium (−).