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Delayed-phase PGD2 generation and induction of PGHS-2 in the presence of arachidonic acid or PGE2. BMMC were stimulated with SCF + IL-10 + IL-1β in the presence of increasing concentrations of PGE2 or arachidonic acid for 1 h, washed, and incubated for 7 h more with cytokines and PGE2 or arachidonic acid to assess delayed-phase PGD2 generation by RIA (A and C) and PGHS-2 induction by SDS/PAGE immunoblotting (B and D).
