Figure 3.

Expression of PNR mRNA in rodents. (A) Northern hybridization analyses of mRNA from rat tissues using the human PNR cDNA as a probe. All lanes contain 2 μg of poly(A)⁺ RNA. β-Actin was used to normalize for RNA quality. (B) In situ hybridization of mouse retina sections with digoxigenin-labeled PNR and CRX RNA probes. An adjacent section was stained with hematoxylin/eosin (HE) to help visualize different cell layers. gcl, ganglion cell layer; ipl, inner plexiform layer; inl, inner nuclear layer; opl, outer plexiform layer; onl, outer nuclear layer; os, outer segments; pe, pigment epithelium.