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Association of Ras-GRF1 with dominant-negative forms of Ha-Ras, Rac1, and RhoA. Proteins within anti-FLAG antibody M2 (+) or control antibody (−) immunoprecipitates from 293 cells transfected with pCMV5-Ras-GRF and expression plasmids for Gβγ and indicated FLAG-tagged small GTP-binding proteins were stained with an anti-Ras-GRF1 antibody. A representative result of three independent experiments is shown.
