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TraR gel retardation assays. (A) Plasmid pJZ304 was digested with HindIII and XbaI and was end-labeled. Binding reactions contained 10−12 M DNA, and TraR (monomer) was added at 75 nM (lane 1), 37.5 nM (lane 2), 18.8 nM (lane 3), 9.4 nM (lane 4), 4.7 nM (lane 5), 2.4 nM (lane 6), 1.2 nM (lane 7), 0.6 nM (lane 8), and 0 nM (lane 9). (B) Hill plot of data obtained in A.
