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Transfection of Sf9 cells with p35 gene construct significantly inhibits H2O2 (HP)-induced cell death. Sf9 cells were transfected with 8–12 μg of pNN1, carrying the p35 gene under the hsp70 transcriptional control, and were incubated for 24 h before being subjected to HP treatment. Cells were scored microscopically for apoptosis 24 h after HP treatment.
