Figure 5.

P35 acts very upstream in preventing oxidative stress-induced apoptosis. (A) p35 is unable to rescue cells from apoptosis after pretreatment with H₂O₂ (HP) Cells were infected with wild-type AcNPV after different time periods of HP treatment and then assayed for apoptosis 24 h after viral infection. The number of apoptosed cells is scored microscopically. (B) Apoptosis was assayed by the generation of nucleosomal DNA ladder formation in cells infected with wild-type AcNPV (Ac) at different time periods after HP treatment. Lane 1, C (control cells); lane 2, cells infected with Ac alone; lane 3, cells treated with HP alone; lanes 4, 5, 6, 7, 8, cells were infected with virus at different time periods (0, 1, 2, 4, 6 h) after HP treatment.