Figure 5.

Smad3 is required for TGFβ-mediated transcriptional activation of 4xSBSMT. Smad3 homozygous null or Smad3 heterozygous mouse primary fibroblasts were transfected with 3 μg of 3TP-Lux, 4xSBSMT, or 4xAP1MT reporter constructs. Twenty hours after transfection, vehicle or 100 pM TGFβ1 was added. Twenty-four hours after treatment, luciferase activity was quantified. The averages of duplicate transfections were used to determine the fold induction by TGFβ over the vehicle control. The 3TP-Lux data is shown as a positive control for the experiment (35).