Figure 3.

Sequence analysis to test the effect of a single C nucleotide inserted into a tetracytidine motif containing editing site IV as the 3′ most cytidine residue. For comparison, editing in the wild-type ndhB sequence as contained in line Nt-pRB59 also is shown. DNA and cDNA samples were amplified with primer pair P11/P28 and sequenced directly with primer P28. Because of the polarity of this primer, the autoradiograph shows the sequences of the DNA strand complementary to the mRNA. Arrowheads mark the editing positions in the cDNA lanes, and roman numerals indicate editing-site numbers.