Effect of the oligomerization state of NtrC on EPR spectral changes at position 86. All proteins (25-μM dimer) carried a spin label at position 86 (D86C*; not designated). Dashed and solid lines indicate spectra for unphosphorylated and phosphorylated forms, respectively, of the proteins indicated (t = 22–23°C). (a) NtrCD86C*. (b) Isolated N-terminal domain of NtrCD86C*. (c) NtrCD86C*, S160F. (d) NtrCD86C*, S160F + enhancer DNA (12.5-μM fragment). (e) NtrCD86C*, Δ444–469 (50-μM monomer).