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Effect of phosphorylation on transcriptional activation by NtrC derivatives. Catalysis of open-complex formation by phosphorylated (+P) and unphosphorylated (−P) forms of spin-labeled NtrCD86C, S160F (D86C*S160F) and NtrCD86C (D86C*) was assessed on plasmid pJES534 (1 nM) in a single-cycle transcription assay as described in Materials and Methods.
