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. 1999 Apr 27;96(9):4886–4891. doi: 10.1073/pnas.96.9.4886

Figure 3.

Figure 3

RB 2121 inhibits NCp7-dependent annealing of tRNA3Lys to HIV-1 (77–257)RNA. Fixed concentrations of [33P]tRNA3Lys and RNA(77–257) (5 × 10−12 mol of each denatured RNA per assay) were incubated in 25 mM Tris⋅HCl, pH 7.5/0.1 mM MgCl2/80 mM NaCl buffer at 37°C in presence of 25 μM, 125 μM, and 250 μM RB 2121 (lanes 2–4) or with 13 μM (12–53)NCp7 in the presence of 0 μM, 100 μM, and 500 μM RB 2121 (lanes 6–8). Lanes 1 and 5 represent tRNA as a control (C). Samples were analyzed by native PAGE at 4°C, and complexes were visualized by autoradiography. h, high molecular weight complex; t, ternary complex made of tRNA3Lys, RNA(77–257), and (12–53)NCp7.