Figure 6.

Selective inhibition by RB 2121 of early and late HIV-1 DNA synthesis. (a) Gel electrophoresis of PCR products. Infected CEM-4 cells were treated by increasing concentrations of RB 2121 for 24 h. DNA was amplified for β-globin-generated (lane 1; 105 bp; as a control), early RT-generated (lane 2; 140 bp), and late RT-generated (lane 3; 293 bp) HIV-1 sequences. The specific dose-dependent inhibition of early and late HIV-1 transcripts by RB 2121 suggests an impairment of RT processivity. (b) Quantitative analysis (±SD) of the PCR results. Inhibition is expressed as percentage of controls (untreated infected cells).