Figure 2.

Orientation of the cleavage plane of transformed human corneal epithelial cells also was affected by applied EFs (strength and polarity as shown). (a) No field control. Cleavage furrows are evident and oriented randomly. (b) Dividing cells in an applied EF showed preferential orientation of the cleavage furrow. The angle formed by the cleavage plane and the vector of the electric field was used to calculate the polarization of cleavage angle (for details see Materials and Methods). The cells were cultured in DMEM/F12 with 15% heat-inactivated FBS.