Figure 3.

Visualization of nitrotyrosine residues and iNOS protein in the lungs of transgenic C57BL iNOS(−/−) and control C57BL iNOS(+/+) mice infected 3 days with 1.5 × 10⁷ cfu per ml M. pulmonis. (A) Nitrotyrosine staining of lungs from resistant C57BL iNOS(+/+) mice. (B) Nitrotyrosine staining from A in the presence of excess nitrotyrosine (10 mM). (C) Nitrotyrosine staining of lungs of C57BL iNOS(−/−) mice. (D) Nitrotyrosine staining from C in the presence of excess nitrotyrosine (10 mM). (E) iNOS staining of BALs from C57BL iNOS(+/+) mice. (F) iNOS staining of BALs C57BL iNOS(−/−) mice. Shown are representative pictures of results, which were reproduced at least twice.