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. Author manuscript; available in PMC: 2008 Jan 8.
Published in final edited form as: Neuron. 2007 Dec 6;56(5):866–879. doi: 10.1016/j.neuron.2007.10.020

Figure 1. Pairing of perforant path and Schaffer collateral inputs to a CA1 neuron induces input-timing-dependent plasticity (ITDP) of the Schaffer collateral EPSPs.

Figure 1

(A) Schematic of experimental setup. Whole-cell recordings were obtained from the soma of CA1 pyramidal neurons. One stimulating electrode was placed in the middle third of stratum radiatum (SR), the site of Schaffer collateral (SC) synapses, and one stimulating electrode was placed in the inner half of stratum lacunosum-moleculare (SLM), the site of perforant path (PP) synapses. (B) EPSPs in response to PP (distal) and SC (proximal) stimulation. Each trace shows the average of several EPSPs. Left (before pairing), PP or SC EPSPs obtained at the end of the baseline period. Middle (during pairing), two superimposed paired PP and SC EPSPs elicited during a −20 ms pairing protocol (SC stimulation occurs 20 ms after PP stimulation). The smaller EPSP pair (dotted trace) was recorded during the middle third of the 90 s, 1 Hz ITDP pairing protocol. The larger EPSP pair was elicited at the end of the pairing protocol (solid trace). Right (after pairing), PP or SC EPSPs elicited after the pairing protocol. Scale bars: 2 mV, 100 ms. (C-D) Time course of the PP (C) and SC (D) EPSPs during the entire experiment. Open symbols show fold change in peak amplitude of individual EPSPs; filled symbols show boxcar averages of fold change in EPSP amplitude during 1 minute (6 stimuli) windows. Arrow indicates start of 90 s pairing protocol.