Figure 1.

Proliferating sporocysts and cercaria produced in vitro. (Top) Daughter sporocyst (DS) and developing daughter sporocyst embryos (DSE) inside a mother sporocyst (MS), cocultured with Bge cells. (Middle) Cercaria produced from proliferating daughter sporocyst culture maintained for 8 months in vitro. (Bottom) Sequestered sporocysts separated from Bge cells by a permeable membrane. Cultures were initiated by transformation of parasitized rodent-derived miracidia and maintained as described in the text. (×150.)