Table 1.

Proliferation of daughter sporocysts in vitro

Culture condition	Time to appearance, weeks	Production in vitro
Bge, 105 cells/well	3	Continuous
Bge, 1.5  ×  104 cells/well	5	Continuous
<10 sporocysts/well	—	0
10–40 sporocysts/well	5	20*
>40 sporocysts/well	3	Continuous
Without Bge	—†	0
Sequestered sporocysts‡	4	Continuous
After in vitro–in vivo passage	4	Continuous

Unless otherwise indicated, cultures were initiated at 10⁵ Bge cells per well and >50 sporocysts per well. Cultures from in vitro–in vivo passage were derived from miracidia collected from a hamster previously infected with cercariae derived from injection of snails with sporocysts from established cultures. Embryogenesis was evident when embryos were seen moving within the bodies of their mothers. Times are approximate. 

^*No daughter sporocysts. 

^†Sporocysts survived 4 weeks. 

^‡Sporocysts separated from Bge cells by a permeable membrane.