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. 1979 Sep;139(3):713–720. doi: 10.1128/jb.139.3.713-720.1979

Genetic and physiological characterization of Pseudomonas aeruginosa mutants affected in the catabolic ornithine carbamoyltransferase.

D Hass, R Evans, A Mercenier, J P Simon, V Stalon
PMCID: PMC218014  PMID: 113384

Abstract

In Pseudomonas aeruginosa arginine can be degraded by the arginine "dihydrolase" system, consisting of arginine deiminase, catabolic ornithine carbamoyltransferase, and carbamate kinase. Mutants of P. aeruginosa strain PAO affected in the structural gene (arcB) of the catabolic ornithine carbamoyltransferase were isolated. Firt, and argF mutation (i.e., a block in the anabolic ornithine carbamoyltransferase) was suppressed specifically by a mutationally altered catabolic ornithine carbamoyltransferase capable of functioning in the anabolic direction. The suppressor locus arcB (Su) was mapped by transduction between hisII and argA. Second, mutants having lost suppressor activity were obtained. The Su- mutations were very closely linked to arcB (Su) and caused strongly reduced ornithine carbamoyltransferase activities in vitro. Under aerobic conditions, a mutant (PA0630) which had less than 1% of the wild-type catabolic ornithine carbamoyltransferase activity grew on arginine as the only carbon and nitrogen source, at the wild-type growth rate. When oxygen was limiting, strain PA0630 grown on arginine excreted citrulline in the stationary growth phase. These observations suggest that during aerobic growth arginine is not degraded exclusively via the dihydrolase pathway.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bartnik E., Weglenski P. Regulation of arginine catabolism in Aspergillus nidulans. Nature. 1974 Aug 16;250(467):590–592. doi: 10.1038/250590a0. [DOI] [PubMed] [Google Scholar]
  2. Haas D., Holloway B. W. Chromosome mobilization by the R plasmid R68.45: a tool in Pseudomonas genetics. Mol Gen Genet. 1978 Jan 17;158(3):229–237. doi: 10.1007/BF00267194. [DOI] [PubMed] [Google Scholar]
  3. Haas D., Holloway B. W., Schamböck A., Leisinger T. The genetic organization of arginine biosynthesis in Pseudomonas aeruginosa. Mol Gen Genet. 1977 Jul 7;154(1):7–22. doi: 10.1007/BF00265571. [DOI] [PubMed] [Google Scholar]
  4. Holloway B. W. Genetics of Pseudomonas. Bacteriol Rev. 1969 Sep;33(3):419–443. doi: 10.1128/br.33.3.419-443.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  6. Legrain C., Stalon V., Noullez J. P., Mercenier A., Simon J. P., Broman K., Wiame J. M. Structure and function of ornithine carbamoyltransferases. Eur J Biochem. 1977 Nov 1;80(2):401–409. doi: 10.1111/j.1432-1033.1977.tb11895.x. [DOI] [PubMed] [Google Scholar]
  7. Legrain C., Stalon V. Ornithine carbamoyltransferase from Escherichia coli W. Purification, structure and steady-state kinetic analysis. Eur J Biochem. 1976 Mar 16;63(1):289–301. doi: 10.1111/j.1432-1033.1976.tb10230.x. [DOI] [PubMed] [Google Scholar]
  8. Matsumoto H., Ohta S., Kobayashi R., Terawaki Y. Chromosomal location of genes participating in the degradation of purines in Pseudomonas aeruginosa. Mol Gen Genet. 1978 Nov 29;167(2):165–176. doi: 10.1007/BF00266910. [DOI] [PubMed] [Google Scholar]
  9. Mee B. J., Lee B. T. An analysis of histidine requiring mutants in Pseudomonas aeruginosa. Genetics. 1967 Apr;55(4):709–722. doi: 10.1093/genetics/55.4.709. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Mindich L., Cohen J., Weisburd M. Isolation of nonsense suppressor mutants in Pseudomonas. J Bacteriol. 1976 Apr;126(1):177–182. doi: 10.1128/jb.126.1.177-182.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Ramos F., Stalon V., Piérard A., Wiame J. M. The specialization of the two ornithine carbamoyltransferases of Pseudomonas. Biochim Biophys Acta. 1967 May 16;139(1):98–106. doi: 10.1016/0005-2744(67)90116-7. [DOI] [PubMed] [Google Scholar]
  12. SHERRIS J. C., SHOESMITH J. G., PARKER M. T., BRECKON D. Tests for the rapid breakdown of arginine by bacteria: their use in the identification of pseudomonads. J Gen Microbiol. 1959 Oct;21:389–396. doi: 10.1099/00221287-21-2-389. [DOI] [PubMed] [Google Scholar]
  13. SLADE H. D., DOUGHTY C. C., SLAMP W. C. The synthesis of high-energy phosphate in the citrulline ureidase reaction by soluble enzymes of Pseudomonas. Arch Biochem Biophys. 1954 Feb;48(2):338–346. doi: 10.1016/0003-9861(54)90349-5. [DOI] [PubMed] [Google Scholar]
  14. Stalon V., Ramos F., Piérard A., Wiame J. M. Regulation of the catabolic ornithine carbamoyltransferase of Pseudomonas fluorescens. A comparison with the anabolic transferase and with a mutationally modified catabolic transferase. Eur J Biochem. 1972 Aug 18;29(1):25–35. doi: 10.1111/j.1432-1033.1972.tb01953.x. [DOI] [PubMed] [Google Scholar]
  15. Stalon V., Ramos F., Piérard A., Wiame J. M. The occurrence of a catabolic and an anabolic ornithine carbamoyltransferase in Pseudomonas. Biochim Biophys Acta. 1967 May 16;139(1):91–97. doi: 10.1016/0005-2744(67)90115-5. [DOI] [PubMed] [Google Scholar]
  16. Stanier R. Y., Palleroni N. J., Doudoroff M. The aerobic pseudomonads: a taxonomic study. J Gen Microbiol. 1966 May;43(2):159–271. doi: 10.1099/00221287-43-2-159. [DOI] [PubMed] [Google Scholar]
  17. Stanisich V. A., Holloway B. W. A mutant sex factor of Pseudomonas aeruginosa. Genet Res. 1972 Feb;19(1):91–108. doi: 10.1017/s0016672300014294. [DOI] [PubMed] [Google Scholar]
  18. Theil E. C., Forsyth G. W., Jones E. E. Expression of the arginine regulon of Escherichia coli W: evidence for a second regulatory gene. J Bacteriol. 1969 Jul;99(1):269–273. doi: 10.1128/jb.99.1.269-273.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Watson J. M., Holloway B. W. Chromosome mapping in Pseudomonas aeruginosa PAT. J Bacteriol. 1978 Mar;133(3):1113–1125. doi: 10.1128/jb.133.3.1113-1125.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Watson J. M., Holloway B. W. Suppressor mutations in Pseudomonas aeruginosa. J Bacteriol. 1976 Mar;125(3):780–786. doi: 10.1128/jb.125.3.780-786.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]

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