Figure 7.

kurtz is required in ORNs for normal olfaction. The avoidance to 0.05% MCH of ORN-rescued krz¹ homozygotes and control genotypes is shown. Both the chromosome 2 (A) and chromosome 3 (B) P{Or83b-Gal4} P elements were used independently to drive krz expression within the ORNs of the krz¹ homozygotes. The level of KURTZ present within P{Or83b-Gal4} (third chromosome)–rescued antenna can be seen in Figure 2D; results were similar for the second chromosome P{Or83b-Gal4} P element. The P{Or83b-Gal4} driver first begins activating transcription. The P{Or83b-Gal4}–rescued krz¹ homozygotes have significantly higher avoidance scores than heat shock–rescued krz¹ homozygotes. The minimum n = 16 for panel (A) and 18 for panel (B). Significant effects of genotype were found in both experiments. The asterisk (*) indicates a significant difference of krz¹ from all the other genotypes according to Bonferroni–Dunn post hoc analysis. These data indicate that KURTZ is required in ORNs for normal MCH sensitivity. The complete genotypes were as follows: krz¹ = P{UASkrz}T12/+; krz¹, P{hspGal4}/krz¹. In panel (A): Or83b; krz¹ = P{UASkrz}T12/P{Or83b-Gal4}; krz¹, P{hspGal4}/krz¹; Or83b; krz¹/+ = P{Or83b-Gal4}/+; krz¹/+; T12/Or83b; krz¹/+ = P{UASkrz}T12/P{Or83b-Gal4}; krz¹, P{hspGal4}/+. In panel (B): OR83b, krz¹ = P{UASkrz}T12/+; krz¹, P{hspGal4}/krz¹, Or83bGal4 (III); OR83b, krz¹/+ = P{UASkrz}T12/+; P{hspGal4}/krz¹, P{Or83b-Gal4}; krz¹/+ = P{UASkrz}T12/+; krz¹/P{hspGal4}.