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. 2008 Jan 16;3(1):e1453. doi: 10.1371/journal.pone.0001453

Figure 1. Proliferation of mouse adipose-derived adult stromal (ADAS) cells in media formulations optimized for neural stem cells.

Figure 1

Cells grow as adherent cultures (A) and upon reaching high confluence they form neurosphere-like clusters (B) in a serum-free media formulation (Media A) which are indistinguishable from neurospheres derived from the subventricular zone in the hippocampus of young adult mice (C); Single-cell derived colonies growing in NeuroCult (Media B) (D) expand to form ring-like structures (E, F); Neural stem cell marker expression in adherent mouse ADAS cells (G–I) and neurospheres (J–L) proliferating in Media A. NSE, Neuron Specific Enolase. Scale bar in A, B, C and F = 220 um; scale bar in D, E G–I = 55 um; scale bar in J–L = 110 um.