Table 1. Expression of neural stem cell markers in mouse ADAS grown in Mesencult and exposed to Neural Induction Medium (NIM).

Marker	Baseline	4 hr NIM	24 hr NIM	48 hr NIM
Nestin	++	+++	++	+
NSE*	+/−	++	+++	+++
GFAP*	+/−	+++	+++	++
Tuj1*	+	++	+++	++

Mouse ADAS cells were proliferated in Mesencult and exposed to NIM for the times indicated prior to harvest in 4% paraformaldehyde for immunocytochemical analyses.

Semi-quantitative assessment of marker expression was performed using the following criteria: +++ = robust expression in greater than 50% of the cells; ++ = moderate expression in greater than 50% of the cells; + = low expression in more than 50% of the cells; +/− = low expression in less than 50% of the cells; − = no detectable expression. Asterisk (*) indicates marker expression confirmed by real-time PCR. Abbreviations: NIM, Neural Induction Medium; NSC, Neural Stem Cell; NSE, Neuron Specific Enolase; GFAP, Glial fibrillary acidic protein; Tuj1, β-tubulin III