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SH3 domain binding of Ena. S2 cells were transfected with Ena under the control of an actin promoter. Proteins retained after solution binding to equal amounts of various GST–SH3 domains were blotted with anti-Ena N-terminal antibodies. None of the proteins bound to the GST negative control (data not shown). Ena binds to the murine and Drosophila Abl SH3 domains, the src SH3 domain, and the C-terminal SH3 domain of Drk.
