Figure 5.

Domain structure and amino acid sequence similarity of HIV-1 Rev to kinesin XKCM1. A shows the locations of Helix 1, the polyproline sequence, Helix 2 (the RNA-binding region with flanking multimerization domains M1 and M2), and the activation domain of Rev. Key residues and positions are numbered above. B shows the XKCM1 motor/catalytic domain sequence from residues 500–550, and aligned Rev sequences below. In Rev, residues are identical (red), very similar (green), or similar (blue) to XKCM1. These residues are bold in XKCM1. Specific residues that are highly conserved in both Rev and the kinesins and that have identified structural and/or functional roles are marked by asterisks. Rev isolates are identified on the right. Rev isolate P04616 (carats) was used in this study.