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. 2000 Jul 24;150(2):321–334. doi: 10.1083/jcb.150.2.321

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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The effect of staurosporine-induced apoptosis on the localization of hICAD in MCF7 cells. MCF7 cells were transiently transfected with plasmids encoding for (top) EGFPF and pcDNA3 or (bottom) procaspase-3 and EGFPF at a molar ration of 4:1. Immunostaining of endogenous hICAD was performed with rabbit anti-ICAD immune serum after exposing the cells to staurosporine (2 μM) for 2 h at 37°C and visualized with laser confocal microscopy. The arrowheads indicate those nuclei that have lost ICAD expression. Bar, 10 μm.