Table 2.
thymidine | ATP | |||||
---|---|---|---|---|---|---|
KM (μM) | kcat (s−1) | kcat/KM (×105 M−1s−1) | KM (μM) | kcat (s−1) | kcat/KM (×104 M−1s−1) | |
wild type | 0.5 ± 0.2 | 0.3 ± 0.08 | 6.0 | 40 ± 12 | 0.4 ± 0.04 | 1.0 |
H53A | 1.2 ± 0.2 | 0.51 ± 0.01 | 4.3 | 86 ± 7 | 0.52 ± .01 | 0.6 |
V34W | 0.9 ± 0.2 | 0.59 ± 0.02 | 6.3 | 23 ± 4 | 0.35 ± 0.01 | 1.5 |
V51W | 0.7 ± 0.1 | 0.25 ± 0.01 | 3.4 | 35 ± 5 | 0.20 ± 0.005 | 0.6 |
G55W | 0.8 ± 0.1 | 0.62 ± 0.01 | 7.7 | 112 ± 10 | 0.45 ± 0.005 | 0.4 |
V58W | 0.5 ± 0.1 | 0.47 ± 0.01 | 9.6 | 29 ± 3 | 0.38 ± 0.008 | 1.3 |
L129W | 3.0 ± 0.3 | 1.91 ± 0.05 | 6.4 | 46 ± 5 | 1.49 ± 0.02 | 3.2 |